Phase contrast darkfield microscopy phase bertrand slider attoarc hbo 100w variable intensity upright fluorescence microscope product overview. It has 3 oil immersion objectives for different applications read more. Only a few hand movements are required to adjust the microscope to the users seated height, head posture, arm length, and hand size. Brightfield, polarization contrast, and lowquality darkfield illumination are available. Leica dm irb dmirb inverted fluorescence microscope predecessor to dmi6000b di8 9216. This includes the examination of samples taken from the human body with a view to providing information on physiological or pathological states or congenital abnormalities, or to determining the. We have this preconfigured with your choice of a binocular, ergo binocular, or trinocular viewing head. The leica dm lb is a research grade upright microscope offering superior stability and excellent image quality for digital imaging.
Therefore one excitation filter for the illumination beam path and two emission filters, one. Manufacturer specifications dmlb, leica microsystems please note. Leica dmlm the leica dmlm is a modern system microscope with a modular design that makes it suitable for a wide range of applications from simple routine examinations in material science to material research tasks. Important notes about this manual this operating manual contains important instructions and information for the operational safety and maintenance of the microscope and accessories. We have set new standards here by introducing our hc optics. None of the equipment posted for sale on is owned by medwow, should you have any questions regarding a specific item, please direct them to the appropriate seller by making use of the available communication channels on the items page.
That means our light microscopes know what matters most. Hbo mercury fluorescence illuminator with variable intensity. This includes the examination of samples taken from the human body with a view to providing information on physiological or pathological states or congenital abnormalities, or to. While most biological microscopes are 18mm, 20mm, or even 22mm, this one has a stunning 25mm field of view. The leica dm750 fluorescence microscope is a high quality upright fluorescence microscope featuring led fluorescence. Leica dm lb research grade microscope climate impacts. Intended purpose of microscopes the leica dm6000 b microscope, to which this user manual belongs, is designed for biological routine and research applications.
This leica cfm 2 user manual is presented in four sections. It applies to the microscope leica dmlb mainly for biological and medical applications and, together with a supplementary manual, to the microscopes dmlp polarized light and dmlm material examinations. Page 17 assembly light sources for incident light fluorescence before assembling to the microscope, check if the leica dm ls microscope can be equipped the lamps have already been inserted fig. Multispectral nearinfrared fluorescence molecular imaging. Fluorescence microscope demo video evos fl duration. The leica dmlb fluorescence microscope can be fully customized to meet the needs of your laboratory. This device and the filter cubes are identical to the corresponding components of leica microscopes for. If the sample contains a significant amount of solvent, put a cover glass on it.
Flexible software modules provide the platform for every cytogenetics and fish. The image below designates the main components of the tc5500 epi fluorescence inverted microscope. It must therefore be kept safely for future reference. Leica dm2500 upright fluorescent microscope manuals. O the leica accessories described in this user manual. The leica dm2500 m will show you how simple and reliable microscopy can be. We have 1 leica dmls manual available for free pdf download. Marketing cm, compound microscopy, product management. The leica dmil is designed for brightfield, phase contrast, integrated modulation contrast imc and incident light fluorescence.
Up for sale is a leica dmlb fluorescent microscope. Dmlb tube eyepieces and objectives the optics are the heart of every microscope and decisive for the quality of information. Leica dm500 user manual safety regulations 14 accessories, maintenance and repair accessories only the following accessories may be used with the leica dm500 microscope. Leica microsystems products are developed with the user in mind. The two fluorescent filter cubes have the following specifications. Browse ads and contact the seller directly or request a quote for more details. Congratulations on purchasing the leica dm100 compound microscope.
Le microscope leica dm ls peut travailler en fluorescence reflechie avec une ampoule attention. Tc5000 series inverted microscope instruction manual. Remove the dust cover from the microscope turn on the mercury lamp a if you need to see the fluorescence through the eye pieces. The information in this manual is subject to modifi cation at. This manual contains important instructions and information on the operating safety and maintenance of the. View online or download leica dmls instructions manual. Las x guides you from the setup to the analysis of the imaging data. Jul 11, 2016 leica dm il fluorescence microscope available from the lab world group. This comes with your choice of hi plan or n plan brightfield objectives. The fluorescence illuminator for the leica dmlb laboratory microscope accommodates up to four filter cubes in horizontal positions in a rotating turret. The leica dmlp pol microscope is the highperformance, universal microscope, not only for polarisation techniques in transmitted and incident light, but also for most conventional light microscopic techniques. Leica dm4000 b leica dm5000 b manual replaceable specimen stage. Placing objects against or covering ventilation slats can lead to a buildup of heat which will.
Olympus ix70 inverted fluorescent microscope manuals. There are no oil objectives available, thus the resolution of the objectives is lower than that of the axioplans. Leica high quality opticshave set global standards in optics engineering. Leica dm irb dmirb inverted fluorescence microscope.
When fluorescence filters are used, the filter protecting from heat emitted by the microscope illuminator must not be removed, since fluorescence filters are sensitive to heat and their function might therefore be impaired. Find online auctions and classified ads for leica fluorescence microscopes on the labx marketplace. The new inverted leica microscope blends ergonomy, a compact design and. The universal illuminator is the heart of the leica dmlm incident light microscope. Once the sample is infocus under dmlb, turn on the microscope camera. Leica dmlb transmitted and reflected light fluorescence. Leica mz6 and dmlb instructions sample preparation place a small amount of sample on a glass slide and spread it out. All microscope techniques and the required accessories for the leica dmil are described and explained in detail in the operation section of this manual, including the associated functions and their use. Minimanual how to use the leica dm2500 microscope transmission light microscopy bf, ph, df x put the sample on the stage holder 12 and move the sample with the xy stage device 8 to the center of the field of view x open the transmission light switch 6 and adjust the intensity of the lamp 3. A leica dm ls microscope instruction manual is included for reference only. Leica db lb research microscope and studo lite imaging.
Keep in mind that the more open a pinhole is, the closer you are approaching basic fluorescence microscopy and losing the confocalness of your scan. Sections were analyzed on a leica dmlb fluorescence microscope leica microsystems, rijswijk, the netherlands equipped with a leica dc300f camera and leicaqwin 2. Used leica dmlb 3015308 microscope microscopy biosurplus. With the help of superresolution fluorescence microscopy, it is even possible to image subresolution structures. Leitz number 5805 lp is a uv violet cube dapi with excitation at 355 425 nm, dichroic mirror 455 nm, and barrier filter at 470 nm. High power 100w halogen transmitted illumination great for phase and darkfield. The leica dmil is the microscope for all requirements in the cell culture lab.
A separate manual is available on cdrom covering the operation of the leica application suite las. Leica dmi8 motorized inverted motorized scanning stage lasers. Leica microsystems fluorescence stereo microscopes use the triplebeam technology, a separate third beam path for fluorescence illumination of the sample without a dichroic mirror. The cytovision platform offers the convenience and comfort of onscreen analysis with flexibility of both software and hardware configurations cytovision is scalable from stand alone capture stations for karyotyping and fish, to fully automated unattended metaphase and cellular fish capture of up to 120 slides. Nuclei were counterstained with dapi, and sections were coverslipped in citifluor mounting medium. Leica dm2500 m microscope user manual general manual. For more information, please visit us at leica dmilm. Brightfield the whole range of objectives from 4x100x magnification can be used for brightfield applications. Permitted uses leica stereo fluorescence systems are equipped with a special fluorescence illuminator. The leica mz fliii fluorescence stereomicroscope offers not only a 3d image but also, compared with a classical microscope at any given magnification, a larger panoramic field of view, more intense fluorescence, and longer working distances.
Leica dmls manuals manuals and user guides for leica dmls. Straightforward setup and control of experiments is becoming more and more important in fluorescence microscopy. Leica dmlb upright research fluorescence microscope lot 718. If your goal is to determine colocalization of multiple. This is a used microscope and sold in asis condition. They are intended for the threedimensional observation of fluorescing objects. You can find the manual book on the desk beside the microscope. Mar 03, 2015 equipped with fluorescence light source no power supply. This models exclusive design features and full range of accessories make it a truly versatile, high quality instrument. A more detailed and thorough account of these procedures can be found in the leica dm6000 b operating manual. Specifications see manual for details research grade microscope with internal light and phase contrast condenser. Upright materials microscope with led illumination leica dm2700 m duration. The microscope of the brand leica type dmlb is ideal for examining and viewing small objects that are not visible to the naked eye. Leica sted and confocal duke light microscopy core facility.
Note that a dense sample may lead to a low quality image. Existing users are not allowed to train other users. The stereo fluorescence modul can be combined with the leica stereomicroscope models ms5, mz6, mz7. Instruction manual leica dmre fluorescence microscope. This microscope is suitable for examining and viewing small objects that are not visible to the naked eye. In such cases the declaration of conformity shall cease to be valid. The fluorescence illuminator for the leica dmlb laboratory microscope. You need a materials microscope system designed for rapid, accurate results. Leica mz6 and dmlb instructions please see gesine for training before your first use. Though the reliability and robustness of leica microscopes is already practically prover. Fluorescence microscopes leica dmlb fluorescence phase.
User manual leica dmi6000 microscope the microscopy alliance. Leica microscope systems are designed to decrease your bench time and provide optimized results. This is a special head that gives a wider field of view, making the image easier to see. Leica dm500 user manual safety regulations 12 information for the person responsible for the instrument information for the person responsible for the instrument o ensure that the leica dm500 microscope is used only by qualified personnel.
A leica dm ls microscope instruction manual is included for. It can be equipped to perform almost any optical technique. The leica dm6000 b microscope, to which this user manual belongs, is designed for biological routine and research applications. User manual leica dmi6000 microscope university of arizona. A universal inverted microscope for routine application. Leica dmlb fluorescence phase contrast super wide field. Home instruments leica dmlb fluorescence microscope. This particular instrument is set up for high quality imaging of epi fluorescence and transmitted light brightfield. O other accessories, provided that these have been expressly approved by leica as being technically safe in this context. This operating manual is an essential component of the microscope, and must be read carefully before the microscope is assembled and put into operation. The fluorescence lamp bulbs are expensive and repeatedly turning them on and off causes them to fail sooner. The leica dmlb offers exemplary stability as a research grade microscope, particularly for photomicrography or digital imaging. Therefore one excitation filter for the illumination beam path and two emission filters, one for each observation beam path, are needed. For phase contrast using the 10x objective turn the condenser disk to show ph1 in front.
With the new leica dm microscope series, leica microsystems has reached its goal of making routine work at the microscope as comfortable and effortless as possible. Leica dmlb fluorescence microscope with jenoptik digital camera. The microscope of zeiss type axiovert 100 is equipped for a long illumination distance. Helene javot, sp5 confocal microscope user manual, updated by mamta srivastava 2 022012, page 1 15 pcic plant cell imaging center bti, room 104b confocal leica sp5 user manual startup procedure.
Lighting a key feature for any comparison microscope, one. This text is a guide to operating the leica fluorescence microscope dm6000. There is a leica manual on a shelf near the microscope. Leica fluorescence microscope the leica mz10 f modular stereomicroscope with 8x80x magnification and 375lpmm high resolution provides triple beam technology, which designates a separate beam path specifically for fluorescent illumination for high contrast and detailed fluorescent imaging. It has many features which make the users daily workload decisively lighter and more convenient. User manual the present user manual describes only the functions of the fluorescence illuminator, the use of the filter sets, and the fitting of the fluorescence module. This manual is an integral part of the leica dmirb microscope and must be read carefully before you start using the microscope. Operating manual axiovert 200 axiovert 200 m inverted.
System solutions consisting of applicationoriented software and hardware components make our instrument even more efficient. Check log sheet to see the notes of previous users. It has been cleaned and lubricated by our firm and is ready to go to work. Log in your bnrf crl fl scopeplate reader session on the fom. There is currently no extra training fee for this microscope. These advantages make it possible to manipulate, sort and process specimens. Mini manual how to use the leica dm2500 microscope transmission light microscopy bf, ph, df x put the sample on the stage holder 12 and move the sample with the xy stage device 8 to the center of the field of view x open the transmission light switch 6 and adjust the intensity of the lamp 3. View and download leica dmls instructions manual online. Leica microsystems dmlb microscope manufacturer specifications. Leica dm il the department of physiology and biophysics. Fluorescence microscopy solutions leica microsystems. Pdf, 4 mb 03 leica dmdm3000 led brochure research en pdf, 8 mb cleaningof microscope optics leicamicrosystems pdf.
Recognized as a leading microscope for pathology, biology, bontany, neuroscience and aquatic studies the leica dm lb can be equipped to handle any imaging task. Includes 10x, 5x objectives, as well as n plan 20x ph1, hcx pl fluotar 40x. The leica dmlb is particularly suitable for photomicrography and digital imaging. Leica dmlb fluorescence microscope with jenoptik digital. Leica dmlb fluorescence microscope microscope central. With unique expertise across the patient journey from tissue acquisition to treatment, leica biosystems is focused on driving innovations by connecting people across radiology, pathology, surgery and oncology leading and breakthrough outcomes for you and your patients. This makes this microscope ideal for use in medical, biological and forensic research. Service manuals karl kaps lamp control lp162 schematics 180 kb download karl kaps som42,52 service manual 2. Leica dmi6000 user manual march 2019 update page 2 leica dmi6000 microscope user policies. The bf brightfield, interference contrast, polarisation, df darkfield and smith quantitative polarisation reflectors and the fluorescence filter systems are easily and conveniently switched with the 4position turret plate. O ensure that this user manual is always available at the leica dm500 microscope. The led fluorescence illumination eliminates the warm upcool down period, bulb changes, and is preset with no centering necessary.
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